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<div><h1>3分鐘學(xué)會(huì)大鼠白介素ELISA試劑盒的使用方法</h1></div> <div id="xdnpptlp7lj1" class="pro_details_tongji"> 　更新時(shí)間：2023-01-13　點(diǎn)擊量：<span id="Click">911</span> </div> <div id="xdnpptlp7lj1" class="n_con" id="c_detail_wrap"> <div>　　<a href="http:///Products-37032381.html" target="_blank"><strong>大鼠白介素ELISA試劑盒</strong></a>的使用方法如下：<br /> </div><div>　　1、取出酶標(biāo)記板，設(shè)置空白孔，在空白孔中依次加入100μL標(biāo)準(zhǔn)品。<br /> </div><div>　　2、分別標(biāo)記樣品編號(hào)，并添加100μL稀釋樣品置于空白微孔中。<br /> </div><div>　　3、將酶標(biāo)記板在37℃孵育30分鐘。<br /> </div><div>　　4、取出酶標(biāo)簽板，扔掉其中的液體，并在每個(gè)孔充滿所用洗滌劑后立即扔掉液體。<br /> </div><div>　　5、在每個(gè)孔填充了應(yīng)用的洗滌劑后，輕輕搖動(dòng)酶標(biāo)記板30秒，從孔中搖動(dòng)應(yīng)用的洗滌劑，并將酶標(biāo)記板拍干在吸水紙上。<br /> </div><div>　　6、重復(fù)步驟55次，并將酶標(biāo)簽板拍干在吸水紙上。<br /> </div><div>　　7、將100加入酶標(biāo)記偶聯(lián)溶液的標(biāo)準(zhǔn)孔和樣品孔μL中。<br /> </div><div>　　8、將96孔板在37℃孵育30分鐘。<br /> </div><div>　　9、取出酶標(biāo)簽板，扔掉其中的液體，并在每個(gè)孔充滿所施加的清潔劑后立即扔掉液體。<br /> </div><div>　　10、在每個(gè)孔重新填充洗滌劑應(yīng)用溶液后，在室溫下輕輕搖動(dòng)酶標(biāo)簽板30秒，然后從孔中搖動(dòng)洗滌劑應(yīng)用溶液，并將酶標(biāo)簽板拍干在吸水紙上。<br /> </div><div>　　11、重復(fù)步驟55次，并將酶標(biāo)簽板拍干在吸水紙上。<br /> </div><div>　　12、將基板A50加入每個(gè)孔μl后立即加入基板B50輕輕搖動(dòng)以混合。<br /> </div><div>　　13、酶標(biāo)記板在37℃黑暗中孵育15分鐘。<br /> </div><div>　　14、向每個(gè)孔中加入50μL終止溶液，輕輕搖動(dòng)并混合。<br /> </div><div>　　15、在酶標(biāo)記上的450nm處測(cè)量OD；顯色后，應(yīng)在15分鐘內(nèi)進(jìn)行測(cè)定。<br /> </div><div>　　16、根據(jù)制備的標(biāo)準(zhǔn)曲線計(jì)算樣品含量。</div> </div> </div> <div id="xdnpptlp7lj1" class="cl"></div> <div id="xdnpptlp7lj1" class="ye">上一條：<a href="/News-1456026.html">猴ELISA試劑盒的操作步驟及其注意事項(xiàng)介紹</a><br />下一條：<a href="/News-1447859.html">一文解讀小鼠抗體ELISA試劑盒的操作步驟</a></div> </div> </div> </div> <div id="xdnpptlp7lj1" class="clearfix"></div> <div class="xdnpptlp7lj1" id="clear"></div>  <footer class="footer"> <div id="xdnpptlp7lj1" class="wrapper w1200"> <div id="xdnpptlp7lj1" class="ft-inner"> <div id="xdnpptlp7lj1" class="leftCon"> <div 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