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</div><div>　　<a href="http:///Products-37033314.html" target="_blank"><strong>小鼠抗體ELISA試劑盒</strong></a>的操作步驟：<br /> </div><div>　　1、使用前，將所有試劑充分混勻。不要使液體產(chǎn)生大量的泡沫，以免加樣時(shí)加入大量的氣泡，產(chǎn)生加樣上的誤差。<br /> </div><div>　　2、根據(jù)待測(cè)樣品數(shù)量加上標(biāo)準(zhǔn)品的數(shù)量決定所需的板條數(shù)。每個(gè)標(biāo)準(zhǔn)品和空白孔建議做復(fù)孔。每個(gè)樣品根據(jù)自己的數(shù)量來(lái)定，能使用復(fù)孔的盡量做復(fù)孔。<br /> </div><div>　　3、加入稀釋好后的標(biāo)準(zhǔn)品50ul于反應(yīng)孔、加入待測(cè)樣品50ul于反應(yīng)孔內(nèi)。立即加入50ul的生物素標(biāo)記的抗體。蓋上膜板，輕輕振蕩混勻，37℃溫育1小時(shí)。<br /> </div><div>　　4、甩去孔內(nèi)液體，每孔加滿洗滌液，振蕩30秒，甩去洗滌液，用吸水紙拍干。重復(fù)此操作3次。如果用洗板機(jī)洗滌，洗滌次數(shù)增加一次。<br /> </div><div>　　5、每孔加入80ul的親和鏈酶素-HRP，輕輕振蕩混勻，37℃溫育30分鐘。<br /> </div><div>　　6、甩去孔內(nèi)液體，每孔加滿洗滌液，振蕩30秒，甩去洗滌液，用吸水紙拍干。重復(fù)此操作3次。如果用洗板機(jī)洗滌，洗滌次數(shù)增加一次。<br /> </div><div>　　7、每孔加入底物A、B各50ul，輕輕振蕩混勻，37℃溫育10分鐘。避免光照。<br /> </div><div>　　8、取出酶標(biāo)板，迅速加入50ul終止液，加入終止液后應(yīng)立即測(cè)定結(jié)果。<br /> </div><div>　　9、在450nm波長(zhǎng)處測(cè)定各孔的OD值。</div> </div> </div> <div id="xdnpptlp7lj1" 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