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target="_blank"><strong>猴ELISA試劑盒</strong></a>的操作步驟：<div>　　</div><div>　　1、加入50ul標(biāo)準(zhǔn)品（Standards）、血清標(biāo)本于相應(yīng)反應(yīng)板孔中。</div><div>　　</div><div>　　2、每孔加入100ul酶聯(lián)物（Conjugate），輕輕混勻30秒（重要），室溫60分鐘。</div><div>　　</div><div>　　3、洗板：甩盡板內(nèi)液體，用蒸餾水或者洗滌液（普通洗滌液，自配）洗滌反應(yīng)板，并去除水滴（在厚疊吸水紙上拍干）；這樣反復(fù)洗滌5次。</div><div>　　</div><div>　　4、每孔加入100ul顯色液，輕輕混勻10秒，室溫20分鐘。</div><div>　　</div><div>　　5、每孔加入50ul終止液（StopSolution）。輕輕混勻30秒；30分鐘內(nèi)在450nm處讀OD值。</div><div>　　</div><div>　　<strong>猴ELISA試劑盒的操作注意事項(xiàng)：</strong></div><div>　　</div><div>　　1、試劑盒從冷藏環(huán)境中取出應(yīng)在室溫平衡15-30分鐘后方可使用，酶標(biāo)包被板開(kāi)封后如未用完，板條應(yīng)裝入密封袋中保存。</div><div>　　</div><div>　　2、濃洗滌液可能會(huì)有結(jié)晶析出，稀釋時(shí)可在水浴中加溫助溶，洗滌時(shí)不影響結(jié)果。</div><div>　　</div><div>　　3、各步加樣均應(yīng)使用加樣器，并經(jīng)常校對(duì)其準(zhǔn)確性，以避免試驗(yàn)誤差。一次加樣時(shí)間控制在5分鐘內(nèi)，如標(biāo)本數(shù)量多，推薦使用排槍加樣。</div><div>　　</div><div>　　4、請(qǐng)每次測(cè)定的同時(shí)做標(biāo)準(zhǔn)曲線，做復(fù)孔。如標(biāo)本中待測(cè)物質(zhì)含量過(guò)高（樣本OD值大于標(biāo)準(zhǔn)品孔孔的OD值），請(qǐng)先用樣品稀釋液稀釋一定倍數(shù)（n倍）后再測(cè)定，計(jì)算時(shí)請(qǐng)最后乘以總稀釋倍數(shù)（×n×5）。</div><div>　　</div><div>　　5、封板膜只限一次性使用，以避免交叉污染。</div><div>　　</div><div>　　6、底物請(qǐng)避光保存。</div><div>　　</div><div>　　7、嚴(yán)格按照說(shuō)明書(shū)的操作進(jìn)行，試驗(yàn)結(jié)果判定必須以酶標(biāo)儀讀數(shù)為準(zhǔn)。</div> 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