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</br>品牌名稱:科培瑞儲存方式:2-8度冷藏 </br>適用范圍:實驗科研，不得用于臨床保質(zhì)期:6個月</br>試劑盒采用雙抗原一步夾心法酶聯(lián)免疫吸附試驗(ELISA)。往預先</p></div> <ul> <li><img src="/skins/529073/images/dot.png" /><span>更新日期：</span>2024-09-30</li> <li><img src="/skins/529073/images/dot.png" /><span>訪問量：</span>444</li> </ul> <div id="xdnpptlp7lj1" class="pro_detail_btn"> <a href="#order" class="prodtl_btn1" rel="nofollow">產(chǎn)品咨詢</a><a href="/contact.html" target="_blank" class="prodtl_btn2" rel="nofollow">聯(lián)系我們</a> </div> </div> <div id="xdnpptlp7lj1" class="clear"></div> </div>  <div id="xdnpptlp7lj1" class="pro_con" id="c_detail_wrap"> <div id="xdnpptlp7lj1" class="pro_con_tlt"><span>詳情介紹</span></div> <link type="text/css" rel="stylesheet" href="/css/property.css"> <script> window.onload=function(){ changeTableHeight(); } window.onresize=function(){ changeTableHeight(); } function changeTableHeight(){ $(".proshowParameter table th").each(function?(i,o){ ????var $this=$(o),? ????height=$this.next().height(); ????$(this).css("height",height); var obj = $(o); var val = obj.text(); if(val == '' || val == null || val == undefined){ $(this).addClass('none'); }else{ $(this).removeClass('none'); } }); $(".proshowParameter table td").each(function?(i,o){ var obj = $(o); var val = obj.text(); if(val == '' || val == null || val == undefined){ $(this).addClass('none'); }else{ $(this).removeClass('none'); } }); } </script> <section class="proshowParameter"> <table style=" margin-bottom:20px;"> <tbody> <tr><th>品牌</th><td>其他品牌</td><th>純度</th><td>99%</td></tr><tr><th>分子量</th><td>酶聯(lián)免疫</td><th>規(guī)格</th><td>48T/96T</td></tr><tr><th>供貨周期</th><td>現(xiàn)貨</td><th>主要用途</th><td>用于科學實驗,不得用于臨床</td></tr><tr><th>應用領域</th><td>醫(yī)療衛(wèi)生,食品,生物產(chǎn)業(yè),制藥,綜合</td><th>品牌</th><td>科培瑞</td></tr><tr><th>規(guī)格</th><td>48T/96T</td><th>儲存條件</th><td>2-8℃冷藏</td></tr><tr><th>試劑盒優(yōu)勢</th><td>靈敏性高、特異性強</td><th>特色服務</th><td>免費代測</td></tr> </tbody> </table> </section> <p><strong><strong>大鼠Ⅰ型前膠原N端前肽 PⅠNP ELISA試劑盒</strong><br/>型號：KPR-DS10668<br/><strong>大鼠Ⅰ型前膠原N端前肽 PⅠNP ELISA試劑盒</strong><br/>檢測試劑盒本試劑盒只能用于科學研究，不得用于醫(yī)學診斷產(chǎn)品參數(shù)規(guī)格:48/96T 檢測方法:酶聯(lián)免疫品牌名稱:科培瑞儲存方式:2-8度冷藏適用范圍:實驗科研，不得用于臨床保質(zhì)期:6個月<br/></strong></p><p><strong>試劑盒采用雙抗原一步夾心法酶聯(lián)免疫吸附試驗(ELISA)。往預先包被病毒性腹瀉病毒抗體的包被微孔中，依次加入標本、HRP標記的檢測抗原，經(jīng)過溫育并洗滌。用底物TMB顯色，TMB在過氧化物酶的催化下轉(zhuǎn)化成藍色，并在酸的作用下轉(zhuǎn)化成終的黃色。用酶標儀在450NM波長下測定吸光度(OD值)，與 CUTOFF值相比較，從而判定標本中豬病毒性腹瀉病毒(BVDV)的存在與否。</strong></p><p><strong><br/><br/><img src="https://img63.chem17.com/gxhpic_4c316a3657/819f05fe9a3ee920b90a76e35599ef590e32eff3f5a6f4672d74b3d12b8a4406e0ad7f353377982c.jpg" alt=""/><br/>上海科培瑞生物科技有限公司經(jīng)過數(shù)年的努力發(fā)展已迅速成為集產(chǎn)品研發(fā)、經(jīng)營為一體的專業(yè)化生物工程公司。公司具有具有完善的實驗技術開發(fā)平臺，成熟的抗原、抗體研發(fā)系統(tǒng)，熟練掌握各種酶聯(lián)技術，結合公司的研發(fā)團隊，可以有效的保證公司產(chǎn)品強的穩(wěn)定性和高的準確性，同時又具有競爭力的價格。公司目前可以提供生化試劑、分子生物學試劑及試劑盒，各種抗體及免疫學試劑盒、實驗耗材等多門類上萬種產(chǎn)品，產(chǎn)品涉及分子生物學、細胞生物學、免疫學等生命科學的各個領域。＃＃<br/>產(chǎn)品有哪幾種規(guī)格？可檢測多少個樣本？</strong></p><p><strong>產(chǎn)品分為48T和96T兩種規(guī)格。</strong></p><p><strong>每次實驗的標準曲線一般設7個不同濃度，加上空白孔，標準曲線一共需要8個孔。因此，一個48T試劑盒多可以測定40個樣本（不做重復且一次用完），一個96T試劑盒多可以測定88個樣本（不做重復且一次用完）。可以根據(jù)實際樣本數(shù)量和實驗計劃選擇合適的產(chǎn)品規(guī)格。</strong></p><p><strong>48T和96T的試劑盒有哪些不同？</strong></p><p><strong>48T和96T的試劑盒，每個孔的反應體系都是*一樣的。不同的是試劑盒中各組分的規(guī)格，詳見說明書。</strong></p><p><strong><br/></strong></p><p><strong>二.方法類型和操作步驟</strong></p><p><strong>雙位點一步法</strong></p><p><strong>在雙抗體夾心法測定抗原時，如應用針對抗原分子上兩個不同抗原決定簇的單克隆抗體分別作為固相抗體和酶標抗體，則在測定時可使標本的加入和酶標抗體的加入兩步并作一步（圖15-5）。這種雙位點一步不但簡化了操作，縮短了反應時間，如應用高親和力的單克隆抗體，測定的敏感性和特異性也顯著提高。單克隆抗體的應用使測定抗原的ELISA提高到新水平。</strong></p><p><strong>在一步法測定中，應注意鉤狀效應（hookeffect），類同于沉淀反應中抗原過剩的后帶現(xiàn)象。當標本中待測抗原濃度相當高時，過量抗原分別和固相抗體及酶標抗體結合，而不再形成夾心復合物，所得結果將低于實際含量。鉤狀效應嚴重時甚至可出現(xiàn)假陰性結果。</strong></p><p><strong><br/>用途</strong></p><p><strong>ELISA的基礎是抗原或抗體的固相化及抗原或抗體的酶標記。結合在固相載體表面的抗原或抗體仍保持其免疫學活性，酶標記的抗原或抗體既保留其免疫學活性，又保留酶的活性。在測定時，受檢標本(測定其中的抗體或抗原)與固相載體表面的抗原或抗體起反應。用洗滌的方法使固相載體上形成的抗原抗體復合物與液體中的其他物質(zhì)分開。再加入酶標記的抗原或抗體，也通過反應而結合在固相載體上。此時固相上的酶量與標本中受檢物質(zhì)的量呈一定的比例。加入酶反應的底物后，底物被酶催化成為有色產(chǎn)物，產(chǎn)物的量與標本中受檢物質(zhì)的量直接相關，故可根據(jù)呈色的深淺進行定性或定量分析。由于酶的催化效率很高，間接地放大了免疫反應的結果，使測定方法達到很高的敏感度。 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