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檢測方法:酶聯(lián)免疫品牌名稱:科培瑞儲存方式:2-8度冷藏適用范圍:實驗科研，不得用于臨床保質(zhì)期:6個月<br/></strong></p><p><strong>試劑盒采用雙抗原一步夾心法酶聯(lián)免疫吸附試驗(ELISA)。往預先包被病毒性腹瀉病毒抗體的包被微孔中，依次加入標本、HRP標記的檢測抗原，經(jīng)過溫育并洗滌。用底物TMB顯色，TMB在過氧化物酶的催化下轉(zhuǎn)化成藍色，并在酸的作用下轉(zhuǎn)化成終的黃色。用酶標儀在450NM波長下測定吸光度(OD值)，與 CUTOFF值相比較，從而判定標本中豬病毒性腹瀉病毒(BVDV)的存在與否。</strong></p><p><strong><br/><strong style="white-space: normal;"><strong>人切除修復交叉互補基因1 ERCC1 ELISA試劑盒</strong></strong><img src="https://img63.chem17.com/gxhpic_4c316a3657/819f05fe9a3ee920b90a76e35599ef590e32eff3f5a6f4672d74b3d12b8a4406e0ad7f353377982c.jpg" alt=""/><br/>上?？婆嗳鹕锟萍加邢薰窘?jīng)過數(shù)年的努力發(fā)展已迅速成為集產(chǎn)品研發(fā)、經(jīng)營為一體的專業(yè)化生物工程公司。公司具有具有完善的實驗技術(shù)開發(fā)平臺，成熟的抗原、抗體研發(fā)系統(tǒng)，熟練掌握各種酶聯(lián)技術(shù)，結(jié)合公司的研發(fā)團隊，可以有效的保證公司產(chǎn)品強的穩(wěn)定性和高的準確性，同時又具有競爭力的價格。公司目前可以提供生化試劑、分子生物學試劑及試劑盒，各種抗體及免疫學試劑盒、實驗耗材等多門類上萬種產(chǎn)品，產(chǎn)品涉及分子生物學、細胞生物學、免疫學等生命科學的各個領域。＃＃<br/></strong></p><p><strong>產(chǎn)品的穩(wěn)定性如何？</strong></p><p><strong>產(chǎn)品在研發(fā)前期已通過嚴格的穩(wěn)定測試，發(fā)貨前亦須通過檢測并質(zhì)檢，在市場上深受廣大客戶的贊許！</strong></p><p><strong>一次ELISA實驗需要多長時間？</strong></p><p><strong>雙抗體夾心ELISA法一次實驗需要4-4.5小時，競爭ELISA法一次實驗需要2-2.5小時。</strong></p><p><strong><br/></strong></p><p><strong>三.方法類型和操作步驟</strong></p><p><strong>間接法測抗體</strong></p><p><strong>間接法是檢測抗體常用的方法，其原理為利用酶標記的抗抗體以檢測已與固相結(jié)合的受檢抗體，故稱為間接法。操作步驟如下：</strong></p><p><strong>⑴將特異性抗原與固相載體連接，形成固相抗原：洗滌除去未結(jié)合的抗原及雜質(zhì)。</strong></p><p><strong>⑵加稀釋的受檢血清：其中的特異抗體與抗原結(jié)合，形成固相抗原抗體復合物。經(jīng)洗滌后，固相載體上只留下特異性抗體。其他免疫球蛋白及血清中的雜質(zhì)由于不能與固相抗原結(jié)合，在洗滌過程中被洗去。</strong></p><p><strong>⑶加酶標抗抗體：與固相復合物中的抗體結(jié)合，從而使該抗體間接地標記上酶。洗滌后，固相載體上的酶量就代表特異性抗體的量。例如欲測人對某種疾病的抗體，可用酶標羊抗人IgG抗體。</strong></p><p><strong>⑷加底物顯色：顏色深度代表標本中受檢抗體的量。</strong></p><p><strong>本法只要更換不同的固相抗原，可以用一種酶標抗抗體檢測各種與抗原相應的抗體。</strong></p><p><strong><br/>用途<p>ELISA的基礎是抗原或抗體的固相化及抗原或抗體的酶標記。結(jié)合在固相載體表面的抗原或抗體仍保持其免疫學活性，酶標記的抗原或抗體既保留其免疫學活性，又保留酶的活性。在測定時，受檢標本(測定其中的抗體或抗原)與固相載體表面的抗原或抗體起反應。用洗滌的方法使固相載體上形成的抗原抗體復合物與液體中的其他物質(zhì)分開。再加入酶標記的抗原或抗體，也通過反應而結(jié)合在固相載體上。此時固相上的酶量與標本中受檢物質(zhì)的量呈一定的比例。加入酶反應的底物后，底物被酶催化成為有色產(chǎn)物，產(chǎn)物的量與標本中受檢物質(zhì)的量直接相關(guān)，故可根據(jù)呈色的深淺進行定性或定量分析。由于酶的催化效率很高，間接地放大了免疫反應的結(jié)果，使測定方法達到很高的敏感度。 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