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　更新時間：2023-11-09　點擊量：<span id="Click">684</span> </div> <div id="xdnpptlp7lj1" class="n_con" id="c_detail_wrap"> <div>　　<strong>人抗體ELISA試劑盒</strong>是一種常用的免疫學實驗方法，用于檢測體內(nèi)是否存在特定人類抗體的定量分析。其原理基于酶聯(lián)免疫吸附法，主要包括固相抗原涂布、樣品孵育、抗體結(jié)合和檢測信號等步驟。<br /> </div><div>　　<a href="http:///Products-37034485.html" target="_blank"><strong>人抗體ELISA試劑盒</strong></a>的基本原理如下：<br /> </div><div>　　首先，將待檢測抗原或抗體固定在微孔板上。這些固相抗原可以是純化的蛋白質(zhì)、多肽、糖類等，或者是細胞濾液、組織提取物等復雜混合物。通過固定，使抗原或抗體與微孔板表面非特異性地結(jié)合。<br /> </div><div>　　隨后，加入待測樣品到微孔中，并讓其充分接觸固相抗原，進行孵育。如果樣品中存在與固相抗原或抗體特異性結(jié)合的抗體，它們將與固相抗原結(jié)合形成抗原-抗體復合物。<br /> </div><div>　　然后，洗滌孔內(nèi)未結(jié)合的物質(zhì)，以去除非特異性結(jié)合的成分。這個步驟能有效減少假陽性結(jié)果的產(chǎn)生，提高試劑盒的特異性。<br /> </div><p align="center"><img src="https://img43.chem17.com/9/20231109/638351336040074101629.png" alt="人抗體ELISA試劑盒" width="372" height="302" align="middle" /></p><p> </p><div>　　接下來，加入與被檢測抗體特異性結(jié)合的檢測抗體，并使其與復合物發(fā)生特異性結(jié)合。這樣，形成了夾心型結(jié)構(gòu)：固相抗原-待測抗體-檢測抗體。再次進行洗滌，以去除未結(jié)合的檢測抗體。<br /> </div><div>　　最后，加入一種與檢測抗體結(jié)合產(chǎn)生顯色反應的底物，一般是染色底物，常見的有TMB或ABTS。底物在酶的作用下發(fā)生化學反應，產(chǎn)生可測量的顏色。<br /> </div><div>　　通過測量反應物顏色的強度，可以定量分析待測樣品中存在的特定人類抗體的濃度。通常使用光密度計或酶標儀對反應顏色進行讀取，并將結(jié)果與已知濃度的標準曲線相比較，計算出待測抗體的濃度。<br /> </div><div>　　<strong>人抗體ELISA試劑盒</strong>廣泛應用于許多領域，包括臨床診斷、生物醫(yī)藥研究和生物工程等。具體應用包括：<br /> </div><div>　　1、感染性疾病的診斷：ELISA可以檢測感染性疾病引起的抗體產(chǎn)生，用于早期感染篩查和疾病診斷。<br /> </div><div>　　2、自身免疫疾病的診斷：ELISA可檢測自身免疫疾病相關(guān)抗體的存在，幫助醫(yī)生進行疾病診斷和監(jiān)測治療效果。<br /> </div> </div> </div> <div id="xdnpptlp7lj1" class="cl"></div> <div id="xdnpptlp7lj1" class="ye"> 上一條：<a href="/Article-3557139.html">人激素ELISA試劑盒的操作步驟，值得一看</a><br />下一條：<a href="/Article-3497614.html">人因子ELISA試劑盒在臨床診斷中的作用和意義</a> </div> </div> </div> </div> <div id="xdnpptlp7lj1" class="clearfix"></div> <div class="xdnpptlp7lj1" id="clear"></div>  <footer class="footer"> <div id="xdnpptlp7lj1" class="wrapper w1200"> <div id="xdnpptlp7lj1" class="ft-inner"> <div id="xdnpptlp7lj1" class="leftCon"> <div id="xdnpptlp7lj1" 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